pick_reference_otus_through_otu_table.py – Reference OTU picking/Shotgun UniFrac workflow.

Description:

This script picks OTUs using a reference-based method and constructs an OTU table. Taxonomy is assigned using a pre-defined taxonomy map of reference sequence OTU to taxonomy. If full-length genomes are provided as the reference sequences, this script applies the Shotgun UniFrac method.

Usage: pick_reference_otus_through_otu_table.py [options]

Input Arguments:

Note

[REQUIRED]

-i, --input_fp

The input sequences

-r, --reference_fp

The reference sequences

-o, --output_dir

The output directory

-p, --parameter_fp

Path to the parameter file

[OPTIONAL]

-t, --taxonomy_fp

The taxonomy map [default: None]

-f, --force

Force overwrite of existing output directory (note: existing files in output_dir will not be removed) [default: None]

-w, --print_only

Print the commands but don’t call them – useful for debugging [default: False]

-a, --parallel

Run in parallel where available [default: False]

Output:

Pick OTUs, assign taxonomy, and create an OTU table against a reference set of OTUs.

pick_reference_otus_through_otu_table.py -i inseqs.fasta -r refseqs.fasta -o out -p qiime_parameters.txt -t taxa.txt

Pick OTUs and create an OTU table against a reference set of OTUs without adding taxonomy assignments.

pick_reference_otus_through_otu_table.py -i inseqs.fasta -r refseqs.fasta -o out -p qiime_parameters.txt