12_11 (alpha release*)
*These reference sequence sets represent de-replicated (clustered) versions (at 99% and 97% sequence similarity) of all fungal rDNA ITS sequences in the current UNITE+INSD (International Nucleotide Sequence Databases: NCBI, EMBL, DDBJ) release of circa 300,000 sequences. The taxonomy mapping files provided here were created from the Index Fungorum ranked classification schema provided by UNITE in association with each sequence in their database. Efforts have been made by UNITE to improve the taxonomic information associated with some of the sequences in their database. The QIIME reference sequence sets linked here have not been subject to any other form of curation (manual or automated) and certainly include incorrectly identified sequences, chimeras, and other problematic sequences. The data provide here are a fungal ITS reference alpha version. Responsibility lies with the user to verify the accuracy of associated taxonomic information as well as quality of sequences in these datasets. Improved fungal rDNA ITS reference sets based on the semi-curated centroids for sequence clusters in the UNITE Global Key Annotations module will soon be available here.|
img-qiime-25oct2012 (alpha release; most recent)
Several of our benchmarking studies make use of mock communities (artifical communities constructed by pooling isolated microorganisms together in known abundances). These studies include Bokulich et al., 2013, Caporaso et al., 2011, and Bokulich et al., 2015 (pre-print). The data used in these studies can be downloaded from mockrobiota, a resource compiling mock microbiome data sets (Bokulich et al., (2016)).