adjust_seq_orientation.py – Get the reverse complement of all sequences

Description:

Write the reverse complement of all seqs in seqs.fasta (-i) to seqs_rc.fasta (default, change output_fp with -o). Each sequence description line will have ‘ RC’ appended to the end of it (default, leave sequence description lines untouched by passing -r):

Usage: adjust_seq_orientation.py [options]

Input Arguments:

Note

[REQUIRED]

-i, --input_fasta_fp

Path to the input fasta file

[OPTIONAL]

-o, --output_fp

The output filepath

-r, --retain_seq_id

Leave seq description lines untouched [default: append ” RC” to seq description lines]

Output:

Example:

Reverse complement all sequences in seqs.fna and write result to seqs_rc.fna

adjust_seq_orientation.py -i seqs.fna